Influence of lignin level of raw material on anaerobic digestion process in reorganization and performance of microbial community

Huge quantum of agricultural crop residue from agrarian economies like India is disposed improperly by open land burning contributing to particulate and gaseous pollutants thereby severely impacting the environment human health. There an overwhelming need for sustainable utilization this misplaced resource as feedstock in biofuel production through anaerobic digestion. Substrate composition influences digestibility substrate underlying microbial plasticity involved In study, inoculum was subjected enrichment presence residues with different lignin content whose influence on biogas production, differential modulation/ reorganization communities explored. BMP assay emphasized inverse proportionality between yield, highest yield 622 mL/g VS added case rice straw (lowest lignin, 16.9%), followed wheat straw—556 (18.12% lignin), sugar cane leaves—451 (21.87% lowest 411 cotton waste (highest 23.97%). Biogas efficiency correlated selection shift key hemicellulolytic (Bacteroides, Clostridium, Prevotella, Thermotoga, Cytophaga) lignocellulolytic (Bacillus, Geobacter, Flavobacterium, Spirochaeta, Klebsiella, Escherichia, Pseudomonas, Burkholderia) genotypes comparison initial inoculum. Study highlighted that variable resulted genomic created a scenario high selectively enriching methanogenic pathways such hydrogenotrophic metagenome lignin) acetoclastic lignin).